1LymeHope, Burlington, ON, Canada, 2Bay Area Lyme Foundation, Portola Valley, CA, United States, 3Department of Obstetrics and Gynecology, McMaster University, Hamilton, ON, Canada, 4Science Division, State University of New York Adirondack, Queensbury, NY, United States, 5Department of Biomedical Sciences, University of North Dakota, Grand Forks, ND, United States, 6Mercy Hospital, Saint Louis, MO, United States, 7Department of Pathology, Division of Microbiology and Immunology, University of Utah, Salt Lake City, UT, United States, 8Department of Molecular Biology, Umeå University, Umeå, Sweden, 9Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, United States, 10Duke University School of Medicine, Durham, NC, United States, 11Department of Psychiatry, Lyme and Tick-borne Diseases Research Center, Columbia University,New York, NY, United States, 12LivLyme Foundation, Denver, CO, United States, 13Rotunda Hospital, Mater Misericordiae University Hospital, University College Dublin, Dublin, Ireland, 14Department of Biology, Mount Allison University, Sackville, NB, Canada, 15School of Systems Biology, George Mason University, Manassas, VA, United States, 16Children’s National Hospital, George Washington University School of Medicine and Health Sciences, Washington, DC, United States, 17LymeLight Foundation, Burlingame, CA, United States, 18Steven & Alexandra Cohen Foundation, Stamford, CT, United States, 19Institute of Parasitology, Biology Center, Czech Academy of Sciences, ˇ Ceské Budˇejovice, Czechia, 20Phelix Research and Development, London, United Kingdom, 21Micropathology Ltd, University of Warwick Science Park, Coventry, United Kingdom, 22Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, United States, 23Department of Microbiology and Immunology, Tulane University Health Sciences, New Orleans, LA, United States

1 Department of Dermatology, University College London Hospitals NHS Foundation Trust, London, UK 2 Department of Dermatology, Guy’s and St Thomas’ NHS Foundation Trust, London, UK 3 University College London Hospitals NHS Foundation Trust, London, UK 4 Department of Virology, Infection Division, University College London Hospitals NHS Foundation Trust, London, UK 5 Specialised Microbiology and Laboratories, South West Regional Laboratory, UK Health Security Agency, Bristol, UK 6 Virus Reference Department, United Kingdom Health Security Agency, London, UK 7 Micropathology Ltd, Coventry, UK 8 Health Services Laboratories, London, UK